Coding

Part:BBa_K2983063

Designed by: Tom Zaplana   Group: iGEM19_Evry_Paris-Saclay   (2019-10-21)


FadX of Jacaranda mimosifolia

This part is an Yarrowia lipolytica codon optimized version of a putative bifunctional fatty acid conjugase / desaturase (FadX) from Jacaranda mimosifolia.

Usage and Biology

This putative bifunctional fatty acid conjugase / desaturase FadX is expected to catalyse the conversion of linoleic acid, C18:2 (9Z,12Z) into jacaric acid, C18:3 (8Z, 10E, 12Z).

The sequence was uncovered following an Exome-sequencing that we performed on both fresh and germinated Jacaranda mimosifolia seeds.

For further details, visit the Dry Lab page of our wiki.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 671
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 7
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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